Equipment at Clinical Genomics Uppsala

Illumina NextSeq 550

• High-Output Kit
  • 4 lane flow cell
  • Yield: up to 120 GB
  • Reads per run: up to 800 M
  • Read lengths: up to 2 x 150 bases
  • Applications: Exomes, broad gene panels
• Mid-Output Kit
  • 4 lane flow cell
  • Yield: up to 40 GB
  • Reads per run: up to 260 M
  • Read lengths: up to 2 x 150 bases
  • Applications: Gene panels

Illumina MiSeq 

• 1 lane flow cell
• Yield: up to 10 GB
• Reads per run: up to 18 M
• Read lengths: up to 2 x 300 bases
• Applications: Small gene panels, bacterial/viral genomes

Illumina MiniSeq

• 1 lane flow cell
• Yield: up to 7 GB
• Reads per run: up to 50 M
• Read lengths: up to 2 x 150 bases
• Applications: Small gene panels, few samples, bacterial/viral genomes

For more information on the different platforms, visit Illumina's website.

Nanopore GridION​

• Yield: up to 10 GB per flowcell; 5 flowcells can be combined to one run.
• Multiplexing up till 96 samples.
• Read length: Dependent on amplicon size or extraction quality for WGS upp till 10 kb.
• Applications: small gene panels, bacterial/ viral genomes, fast sequencing, metagenomics

For more information, visit Oxford Nanopore Technologies' website.

NanoString nCounter® FLEX

• Gene expression panels of up to 770 genes
• 12 samples analyzed per run
• Applications: Gene expression analyses

For more information, visit Nanostring's website.

​Bio-Rad QX200™ Droplet Digital™​ PCR system

• Droplet digital PCR analysis in 96-well plates
• Applications: Sensitive detection of specific mutations

Read more about Droplet Digital PCR on Bio-Rad's website.

Mission Bio Tapestri® platform

• Pre-designed or custom DNA and antibody panels
• Applications: Simultaneous detection of SNVs, CNVs and cell surface proteins in single cells

For more information, visit Mission Bio’s website.

Other equipment

Through collaboration with the SNP&SEQ Technology Platform and Uppsala Genome Center we have access to additional instruments, including e.g. NovaSeq 6000 (Illumina) and PacBio instruments.